A mammalian cell-based screening assay to identify

专利名称：A mammalian cell-based screening assay to

identify inhibitors of alphaviruses

发明人：Vidalain, Pierre-Olivier,Tangy, Frédéric,Jacob,

Yves,Lucas-Hourani, Marianne

申请号：EP07291429.4申请日：20071130公开号：EP2065476A1公开日：20090603

摘要：The current invention concerns a method to identify modulators of virulence ofan comprising the steps of i) expressing an non-structural protein 2 (nsP2) codingsequence in at least one subject mammalian cell ; (ii-a) introducing a first reporter geneunder the transcriptional control of an inducible promoter into said at least one subjectmammalian cell ; (iii) monitoring the activity of said first reporter gene ; (iv) comparing saidactivity of said first reporter gene of step (iii) to at least one control mammalian cell notexpressing said nsP2 coding sequence and comprising said first reporter gene under thetranscriptional control of said inducible promoter

申请人：INSTITUT PASTEUR,Centre National de la Recherche Scientifique

地址：25, rue du Docteur Roux 75015 Paris FR,3, rue Michel Ange 75016 Paris FR

国籍：FR,FR

代理机构：Thomas, Dean

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